Genetic improvement of cassava starch quality is an important goal for breeders, especially in changing the ratio of amylose to amylose. Traditional breeding of improved cassava is difficult, and this can be achieved quickly with the help of genome editing techniques. Previous studies have found that SBE2 is the target gene for the production of high amylose cassava. To this end, our center uses the dual sgRNA CRISPR/Cas9 system to carry out gene editing of the cassava SBE2 gene. In the mutants obtained, homozygous mutations or biallelic mutations for the second and fifth exons of SBE2 have the depletion of the SBE2 protein. The amylose and resistant starch content of these mutants were significantly higher than those of the wild-type control, and the physicochemical properties and structure of starch gelatinization characteristics, chain length distribution, and crystallinity in the storage roots were changed. The study shows that CRISPR/Cas9-mediated starch biosynthetic gene mutations in cassava starch are an effective way to breed new varieties of starch with food and industrial application value. The results were recently published online under the title "Editing of the starch branching enzyme gene SBE2 generates high-amylose storage roots in cassava" in Plant Molecular Biology Journal, and contributed to a special collection of "Molecular Regulation of Starch Metabolism".